Summary
[Wormbase] The dpy-30 gene encodes an essential nuclear protein required for hermaphrodite dosage compensation as well as normal male development; DPY-30 is expressed ubiquitously throughout development and can be detected in two different multiprotein complexes: the SDC complex involved in dosage compensation and a second complex containing NOX-1 (a WD repeat-containing protein) and ASH-2 (a trithorax-related protein) that is homologous to the yeast Set1p complex involved in epigenetic regulation of transcription.
Wormbase predicts one model.
AceView synopsis, each blue text links to tables and details
According to AceView, this gene is expressed at very high level, 5.0 times the average gene in this release, mostly in embryos, and some at all stages of development [Kohara cDNAs]. The expression profile for the gene, derived from the proportion of animals at each stage in each Kohara library is: embryos 60%, L1 or L2 larvae 16%, L3 to adult (including dauer) 23%. See the in situ hybridization pattern in Kohara NextDB. The sequence of this gene is defined by 44 cDNA clones and 22 elements defined by RNA-seq, some from embryo (seen 23 times), l1 (3), l2 (3), mixed (2), dauer (once), l4 (once). We annotate structural defects or features in 4 cDNA clones.
The gene contains 1 gt-ag intron. Transcription produces one mRNA. Variant a is transpliced to SL1. Function: There are 11 articles specifically referring to this gene in PubMed. In addition we point below to 17 abstracts. This essential gene is associated to a phenotype (DumPY : shorter than wild-type, uncoordinated locomotion, sluggish, shared oogenic and spermatogenic protein copurified with chromatin). This protein appears to interact with other proteins (3G340, 4N366, 5F105, ASH-2, ZIM-1, ZIM-3). The gene interacts with 3 other genes (DPY-26, HER-1, XOL-1).
The spliced mRNA putatively encodes a good protein, containing Dpy-30, conserved site domain [Pfam].
Please quote: AceView: a comprehensive cDNA-supported gene and transcripts annotation, Genome Biology 2006, 7(Suppl 1):S12. Map on chromosome V, links to other databases and other names Map: This essential gene dpy-30 maps on chomosome V at position +3.85 (interpolated). In AceView, it covers 0.67 kb, from 12191496 to 12192161 (WS190), on the direct strand. Links to:WormBase, NextDB, RNAiDB. Other names: The gene is also known in Wormgenes/AceView by its positional name 5M671, in Wormbase by its cosmid.number name ZK863.6, in NextDB, the Nematode expression pattern database, as CEYK2336. Closest AceView homologs in other species ? The closest human gene, according to BlastP, is the AceView gene MEMO1. The closest mouse gene, according to BlastP, is the AceView gene 2810410M20Rik
Please choose between the zoomable GIF version., and the HTML5/SVG version.
This diagram shows in true scale the gene on the genome, the mRNAs and the cDNA clones.
Alternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron, the small numbers show the support of the introns in deep sequencing (with details in mouse-over) . Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript. Read more...
Mouse over the ending of each transcript gives tissues from which the supporting cDNAs were extracted. Details on tissue of origin for each intron and exon is available from the intron and exons table.
Click on any transcript to open the specific mRNA page, to see the exact cDNA clone support and eventual SNPs and to get details on tissues, sequences, mRNA and protein annotations. Proteins supported by a single continuous cDNA sequence lead to underlining the name/ending of the variant. Names not underlined result from cDNA concatenation in the coding region and should be experimentally checked.
Introns are depicted by broken lines; the height of the top of each intron reflects the relative number of clones supporting this intron. ]^[ A pink broken line denotes an intron with standard boundaries (gt-ag or gc-ag) that is exactly supported (i.e. a cDNA sequence exactly matches the genome over 16 bp, 8 on both sides of the intron). ] ^ ] A blue broken line denotes non-standard introns, exactly supported, but with non-standard at-ac or any other boundaries. ]-[ Pink and ] - ] blue straight lines represent 'fuzzy' introns of the standard and non-standard types respectively, those introns do not follow the 16 bp rule. Black straight lines ]-[denote gaps in the alignments.
Exons: Wide filled pink areas represent putative protein coding regions, narrow empty pink boxes represent the 5'UTR (on the left) and 3' UTR (on the right). Flags identify validated endings: cap site on the 5' side, polyadenylation site on the 3' side. Filled flags correspond to frequent events while empty flags have lesser supporting cDNAs (yet all are validated); at the 3' side, black flags are associated to the main AATAAA signal, blue flags to any single letter variant of the main . More explanations are given in the gene help file
The mRNAs diagrams with the aligned cDNA sequence accessions and their mismatches are available in the mRNA pages accessible from the tab at the top of the page, or here:
In Flash: .a.
or in GIF: .a
Please see these 11 articles in PubMed.
In addition we found 17 papers for which we do not have a PubMed identifier
pm19123269
[wbg12.4p53] xol-1 Transcript Analysis and New 'Alleles'
[wm93p204] Dissection of the dosage compensation process: genetic and molecular analysis of dpy-30, another piece of the puzzle.
[wbg13.1p60] Is Differential Histone H4 Acetylation Involved in the Mechanism of DosageCompensation?
[wbg13.1p25] Analysis of Maternal and Zygotic Gene Expression During Embryogenesis Usingin situ Hybridization
[wbg13.3p39] DPY-27: A Protein Required For Dosage Compensation is Associated With the X Chromosome in XX Animals
[wm95p163] CLONING THE DOSAGE COMPENSATION GENE dpy-21.
[wm95p84] The cell death gene ced-4 encodes both death-promoting and death-preventing transcripts.
[wm95p54] A MOLECULAR MECHANISM FOR X CHROMOSOME DOSAGE COMPENSATION IN C. elegans
[wbg14.1p27] DPY-26 is a Novel Protein Associated with the X Chromosomes in XX Animals
[wbg14.1p92] The cell death gene ced-4 encodes both death-promoting and death-preventing transcripts.
[wcwm96p25] A PROTEIN COMPLEX INCLUDING DPY-26, DPY-27 AND A MITOTIC CHROMOSOME CONDENSATION PROTEIN HOMOLOG IMPLEMENTS DOSAGE COMPENSATION IN C. ELEGANS
[wcwm96p127] DPY-26 ACTS WITH DPY-27 AS A MEMBER OF A DOSAGE COMPENSATION COMPLEX AND INDEPENDENTLY IN MEIOTIC CHROMOSOME SEGREGATION
[wcwm96p24] sdc-2 ACTS AS A SWITCH TO ACTIVATE DOSAGE COMPENSATION AND HERMAPHRODITE DIFFERENTIATION IN XX ANIMALS
[wcwm96p93] Cloning the dosage compensation gene, dpy-21
[wm97ab99] Cloning the dosage compensation gene, dpy-21
To mine knowledge about the gene, please click the 'Gene Summary' or the 'Function, regulation, related genes ' tab at the top of the page. The 'Gene Summary' page includes all we learnt about the gene, functional annotations of neighboring genes, maps, links to other sites and the bibliography. The 'Function, regulation, related genes ' page includes Diseases (D), Pathways, GO annotations, conserved domains (C), interactions (I) reference into function, and pointers to all genes with the same functional annotation.
To see the mRNA diagram, sequence and annotation, click the 'mRNA' tab. To examine expression data from all cDNAs clustered in this gene by AceView, click the 'Expression tissue'.
If you know more about this gene, or found errors, please share your knowledge. Thank you !
